Coding

Part:BBa_K3331004

Designed by: Wang Weizhe   Group: iGEM20_XJTU-China   (2020-10-20)
Revision as of 21:59, 26 October 2020 by Cycling (Talk | contribs)


P.A. pgmA

This part catalyzes the interconversion of alpha-D-glucose 1-phosphate and D-glucose 6-phosphate. This part comes from the genome of Pseudomonas aeruginosa. We optimized the sequence with the codon preferred by Bacillus subtilis. In this way, it can be more efficiently expressed in Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 672
    Illegal BglII site found at 705
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 27
    Illegal AgeI site found at 261
    Illegal AgeI site found at 900
    Illegal AgeI site found at 1278
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

We cloned and confirmed the target fragment by PCR. The length of our fragment is 981bp. The following results showed the successful result.

Figure 1:PCR confirmation of the target fragment.
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Parameters
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