Part:BBa_K2957000

IL-8 (CXCL8)

Contents: IL-8 (CXCL-8), with Spec resistance

What is it?: A Basic part; in this particular case we call it a pL0-G (through Modular Cloning Golden Gate Type II Assembly)

What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.

How to use it?: You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the CDS/gene part which is in a pL0-G backbone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 244
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

MIT_MAHE 2020

Summary

Interleukin-8 (IL-8) is a cytokine unique in its property of target specificity towards neutrophils, with only weak effects on other blood cells. It is produced by various tissues and blood cells. In the inflammatory region, it attracts and activates neutrophils which responds by migration of the cells, the release of granule enzymes, and other intra- and extracellular changes. IL-8 is a member of the Interleukin-8 supergene family that includes other small chemotactic peptides with structural homology.

References

1. Bickel M. (1993). The role of interleukin-8 in inflammation and mechanisms of regulation. Journal of periodontology, 64(5 Suppl), 456–460.