Part:BBa_K3504018
P2A
Part Description
2A self-cleaving peptides is a class of 18–22 aa long peptides that can induce ribosomal skipping during translation of a protein in a cell.
Usage
Characterization
P2A have been shown to have superior cleavage ability when compared to different 2A peptide.
Improvements
We improved the part BBa K1537016 by Inserting GSG and SGS linkers, V5 epitope tag (GKPUPNPLLGLDST) and 3xFlag epitope tag immediately preceding 2A has been shown to improve 2A cleavage efficiency
References
1.Szymczak AL, Workman CJ, Wang Y, Vignali KM, Dilioglou S, Vanin EF, Vignali DA. Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nat Biotechnol 2004; 22:589-94; PMID:15064769; http://dx.doi.org/10.1038/nbt957
2.Yang S, Cohen CJ, Peng PD, Zhao Y, Cassard L, Yu Z, Zheng Z, Jones S, Restifo NP, Rosenberg SA, et al. . Development of optimal bicistronic lentiviral vectors facilitates high-level TCR gene expression and robust tumor cell recognition. Gene Ther 2008; 15:1411-23; PMID:18496571; http://dx.doi.org/10.1038/gt.2008.90
3.Tan YP, Liang HR, Chen AE, Guo XF. Coexpression of double or triple copies of the rabies virus glycoprotein gene using a 'self-cleaving' 2A peptide-based replication-defective human adenovirus serotype 5 vector. Biologicals 2010; 38:586-93; PMID:20682459; http://dx.doi.org/10.1016/j.biologicals.2010.06.001
4.Chng, J., Wang, T., Nian, R., Lau, A., Hoi, K. M., Ho, S. C., ... & Yang, Y. (2015, March). Cleavage efficient 2A peptides for high level monoclonal antibody expression in CHO cells. In MAbs (Vol. 7, No. 2, pp. 403-412). Taylor & Francis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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