Regulatory
Part:BBa_K3505016
Designed by: Asteria Tsapadikou,Emmanouil Stylianakis Group: iGEM20_Thessaly (2020-10-20)
araC-ParaBAD:RBS GB compatible with A1-B1
long
Usage and Biology
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 BBa_K3505007 and has overhangs compatible for Golden Braid cloning. The CDS has position A1-B2
Verification of Cloning
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1148
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 983
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 965
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Categories
Parameters
None |