Protein_Domain
Part:BBa_K3582023
Designed by: Avadhoot Jadhav Group: iGEM20_IISER-Pune-India (2020-10-20)
Cyclotide Kalata B1 domain with strep II tag
The biobrick codes for the cyclotide kalata B1 protein domain consisting of the first 5 loops of the cyclotide construct. This part has been created to allow insertion of peptide sequences in the 6th loop.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 29
References
- 1] Cyclotides, a novel ultrastable polypeptide scaffold for drug discovery. (2011, December 1). PubMed
Central (PMC). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3330703/
- 2] Poth, A. G. (2013, May 7). Cyclotides as Grafting Frameworks for Protein Engineering and Drug Design
Applications. PubMed. https://pubmed.ncbi.nlm.nih.gov/23893608/
- 3] 2. Camarero, J. A. (2019, April 19). The Potential of the Cyclotide Scaffold for Drug Development.
MDPI. https://www.mdpi.com/2227-9059/7/2/31/htm#B31-biomedicines-07-00031
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Categories
Parameters
None |