RNA

Part:BBa_K3328008:Experience

Designed by: Chaoxin Chen   Group: iGEM20_OUC-China   (2020-10-03)
Revision as of 13:26, 20 October 2020 by Hester (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3328013

Compared with the blank control (IPTG=0 M, aTc=0 mg/ml), the fluorescence of GFP was low when only the promoter before toehold sequence was turned on (IPTG=0.1 M, aTc=0 mg/ml). This indicates that the toehold has the advantage of low leakage. When the trigger was expressed (IPTG=0.1 M, aTc=0.25 mg/ml), it showed a high GFP fluorescence of up to 32.3-fold due to the destruction of toehold hairpin structure.

User Reviews

UNIQ165108743b1b6df2-partinfo-00000000-QINU UNIQ165108743b1b6df2-partinfo-00000001-QINU