Coding

Part:BBa_K3468087:Design

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-20)
Revision as of 11:10, 24 October 2020 by YuanWang (Talk | contribs)

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PETase S141P


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Reading the literature found that the i+1 position of β-turn has an obvious residue preference for pro, and Ser141 is at the i+1 position of β-turn. The introduction of proline mutations can theoretically improve thermal stability.


Source

Ideonalla sakaiensis

References