Plasmid

Part:BBa_K3505009

Designed by: Venetios Michelioudakis   Group: iGEM20_Thessaly   (2020-10-20)
Revision as of 15:24, 23 October 2020 by Venetios (Talk | contribs)


pDGB1 alpha 2 (α2) for Bacterial GoldenBraid Level a vector for Golden Braid Cloning. Having cargo LacZa for blue white screening between BsaI and BsmBI sites.Resistance in Kanamycin and ori pBBR1 for multiple protein expression capacities.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3224
    Illegal XbaI site found at 3251
    Illegal PstI site found at 3263
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3224
    Illegal PstI site found at 3263
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3224
    Illegal BamHI site found at 3245
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3224
    Illegal XbaI site found at 3251
    Illegal PstI site found at 3263
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3224
    Illegal XbaI site found at 3251
    Illegal PstI site found at 3263
    Illegal NgoMIV site found at 1291
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3463
    Illegal BsaI.rc site found at 2881


[edit]
Categories
//awards/part_collection
//plasmid/construction
//plasmid/expression
Parameters
None