Part:BBa_K3425021
iGEM Type IIS standard Level 1 Dummy (TU-DY)
Type IIS iGEM standard allows for fast and efficient construction of transcriptional units (TU) and multi-transcriptional units (MTU) in groups of four components. These components are needed in Level 1 assemblies (promoter, RBS, CDS and terminator) but might not be needed in higher level assemblies if the amount of transcriptional units in your system is not a multiple of four. For this reason, placeholders called dummy parts were created based on an existing Modular Assembly method [1]. These parts mimic a basic Type IIS part or a transcriptional unit and can be included in the assembly whenever needed. This page corresponds to TU-DY which was cloned to all pOdd plasmids (pSB1K0#) and mimics transcriptional units. Not present in the part would be BsaI recognition sites, which are positioned so the fusion sites are cut in the proper way. The part with these sites can be found in Team Uppsala 2020's Parts page. More information about this cloning standard and dummy parts can be found in Team Uppsala 2020's iGEM Type IIS standard guidebook
Usage and Biology
Experiments with cloning the original dummy sequences TU-DY (BBa_K3425021) and MTU-DY (BBa_K3425022) together (BBa_K3425064 and BBa_K3425065) showed that these parts should be longer than 4bp and different from each other. This is reflected in the design of the new set of dummies, where each dummy is 8bp long and all of them are different.
| Registry Number | Name | Mimics |
|---|---|---|
| BBa_K3425023 | pSB1K01-DY | TU1 |
| BBa_K3425024 | pSB1K02-DY | TU2 |
| BBa_K3425025 | pSB1K03-DY | TU3 |
| BBa_K3425026 | pSB1K04-DY | TU4 |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
//collections/typeiis/uppsala/placeholders
| None |