Part:BBa_K3351004

HD5, an antimicrobial peptide

Summary

A human antimicrobial peptide with 32 amino acids.HD5 fights against Gram-negative bacteria by disrupting and damaging the membrane and penetrating into the cytoplasm. Different oligomeric states were found to perform different degrees of antimicrobial activity. In solution, dimeric and tetrameric states are generally observed. A dimer was found to be the most active form, whereas a tetramer is the largest stable found in solution.
Moreover, HD5 also shows anti-endotoxin properties by binding to bacterial lipopolysaccharide (LPS) resulting in LPS neutralization. Both dimer and tetramer seem to induce the LPS membrane disruption.

Characterization

Figure.1 growth curve

Reference

[1] Awang T, Pongprayoon P. The adsorption of human defensin 5 on bacterial membranes: simulation studies. J Mol Model. 2018 Sep 5;24(10):273. doi: 10.1007/s00894-018-3812-7. PMID: 30187138. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]