Part:BBa_K3506007

CLB2 promoter

This part is the CLB2 promoter which is -829 to -365 nt relative to CLB2 ATG on the genome of Saccharomyces cerevisiae BY4741. CLB2 promoter is the promoter of the G2/mitotic-specific cyclin-2(CLB2) which accumulates steadily during G2 and is abruptly destroyed at mitosis. CLB2 interacts with the CDC2 protein kinase to form maturation-promoting factor(MPF) and is essential for the control of the cell cycle at the G2/M (mitosis) transition.Previous studies have revealed that CLB2 promoter can regulate the transcription of its downstream gene periodically. Under the control of CLB2 promoter, the downstream gene starts to transcript in the S phase, reaches a peak at the G2/M transition, and rapidly degrades at the end of the M phase.

Usage

This part can be used to couple the transcription of the target gene with yeast cell cycle.CLB2 promoter is a promoter which promotes transciption in late S and G2/M of the yeast cell cycle .If you want to express target gene during cell division,you can place the Clb2 promoter sequence before your gene sequence on the yeast plasmid. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 229
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 203
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]