Device
Part:BBa_K3629012
Designed by: Sravya Kakumanu Group: iGEM20_Calgary (2020-10-18)
T. reesei CBHII expression construct with gibson homology sequences and FLAG tag
Usage and Biology
Design
The native signal peptide from T. reesei was removed so it would not interfere with the fused Lip2 secretion tag native to Y. lipolytica.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2737
Illegal EcoRI site found at 2561 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2561
Illegal NheI site found at 75
Illegal SpeI site found at 2738
Illegal PstI site found at 2752
Illegal NotI site found at 7
Illegal NotI site found at 2745 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2561
Illegal BamHI site found at 2686
Illegal XhoI site found at 124
Illegal XhoI site found at 1198 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2738
Illegal EcoRI site found at 2561 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 2561
Illegal XbaI site found at 16
Illegal SpeI site found at 2738
Illegal PstI site found at 2752
Illegal NgoMIV site found at 1458 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |