Terminator
Part:BBa_K3629004
Designed by: Sravya Kakumanu Group: iGEM20_Calgary (2020-10-11)
Yarrowia lipolytica XPR2 terminator
Terminator sequence from the Yarrowia lipolytica alkaline extracellular protease XRP2 gene.
Usage and Biology
Design
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 271
Illegal EcoRI site found at 265 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 265
Illegal SpeI site found at 272
Illegal PstI site found at 286
Illegal NotI site found at 7
Illegal NotI site found at 279 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 265 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 272
Illegal EcoRI site found at 265 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 265
Illegal XbaI site found at 16
Illegal SpeI site found at 272
Illegal PstI site found at 286 - 1000COMPATIBLE WITH RFC[1000]
References
[edit]
Categories
Parameters
None |