DNA

Part:BBa_K3593010

Designed by: Wanning He   Group: iGEM20_GreatBay_SCIE   (2020-10-08)
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ssDNA, capture probe of control line in method without RPA

This ssDNA can be immobilized on the control line region of test strip. It is complementary with the best 1 aptamer conjugated to a reporter Au nanoparticle, thus reporting a red color to verify the function of lateral flow construction

Sequence and features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterisation of this part

Design

Besides aptamers, we have some short single stranded DNA known as 'probes', which binds to other single stranded DNA. BBa_K3593010 is one of those so-called 'probes' in our design, and play important roles in our hardware, in which it binds and immobilizes the aptamer. Its 5' end is conjugated to biotin[1] to bind with streptavidin.

References

1.Jauset-Rubio M, Svobodová M, Mairal T, et al. Aptamer Lateral Flow Assays for Ultrasensitive Detection of β-Conglutin Combining Recombinase Polymerase Amplification and Tailed Primers. Anal Chem. 2016;88(21):10701-10709. doi:10.1021/acs.analchem.6b03256



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