Regulatory

Part:BBa_K3487007

Designed by: Jianhua Zhou   Group: iGEM20_SZPT-CHINA   (2020-10-09)
Revision as of 08:39, 20 October 2020 by Linruixin (Talk | contribs)


Lysine riboswitch

Description

Riboswitches are metabolite-sensitive elements found in mRNAs that control gene expression through a regulatory secondary structural switch.RS is a Lysine riboswitch that Regulated by lysine. The following is the control principle diagram

T--SZPT-CHINA--RS.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 284
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 256

2020 SZPT-CHINA

Result

We put the lysine riboswitch connected sfGFP engineered bacteria in different concentrations of lysine for 24 hours, and then tested the fluorescent expression of the bacteria. The results are shown in the following.

T--SZPT-CHINA--RS2.png

The results show that the bacteria are in the lysine at a concentration of 30μmol/mL. Fluorescence expression was significantly inhibited, indicating that the lysine riboswitch can work normally.

References

Garst AD, Héroux A, Rambo RP, Batey RT. Crystal structure of the lysine riboswitch regulatory mRNA element. J Biol Chem. 2008;283(33):22347-22351.

[edit]
Categories
//rna/riboswitch
Parameters
None