Composite

Part:BBa_K3431021

Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang   Group: iGEM20_CSMU_Taiwan   (2020-09-08)
Revision as of 16:51, 23 October 2020 by IrisRChen (Talk | contribs)


op21_A_ToeholdSwitch-Regulated Invertase

Description

Toehold Switch with Invertase as Expression Protein (op21_A) is an RNA-based device that is to apply as a biosensor for miRNA. This biosensor is designed to detect and reflect the amount of miRNA-21 through the expression of Beta-Fructosidase (Thermotoga Maritima MSB8) (BBa_K3431000), which can convert sucrose into glucose in a time-saving process and its result can be easily represented in the readout of glucose meter. The mechanism for detection relied on the following part - Toehold Switch for miRNA-21 (op21_A) (BBa_K3431005) - whose restriction on the expression of invertase can be liberated upon binding with miRNA-21. Furthermore, we include T7 promoter (BBa_I719005) and terminator (BBa_K731721) to ensure that our device can transcribe and translate in the environment of PURExpress in vitro protein synthesis kit.

Experiment result


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1425
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1196
    Illegal BamHI site found at 1326
    Illegal XhoI site found at 1397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 997
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None