Part:BBa_K3633000

Coding sequence for tyrosinase from B. megaterium

Description

Tyr gene provides instructions for making enzyme tyrosinase which is located in melanocytes in our skin. Melanocytes are responsible for producing melanin. Melanin is responsible for giving our skin and hair color. It includes eumelanin and pheomelanin. Eumelanin usually shows darker color than pheomelanin. As shown below, tyrosinase is responsible for the first step in melanin production.

Tyrosine is one of the 20 standard amino acids present in the body and used by cells to synthesize proteins. This is a non-essential amino acid, meaning that when it is not only ingested in one's diet, it is also synthesized by the body. Tyrosine is found in casein, a protein in milk and other milk-based products (like cheese). Tyrosine is also present in non-dairy foods that are aged, including some types of meats and red wine.

Experiment & Results

Expression of melanin in E.Coli and vibrio natriegens

The production of melanin by E. coli and V. natriegens at 37℃ was faster than production at 25℃ at first, but the amount of production in V. natriegens at 37℃ was exceed by that in E. coli at 25℃.

Hair dye using synthesized melanin

We first incubated the hair in pH=9 Ca(OH)2 at 50℃ for 40 min. Next, we added the melanin bacterial solution and kept at 50℃ for another 40 min. We successfully dyed hair with our synthesized pigment.

Sequence & Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 320
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]