Coding

Part:BBa_K3502000:Design

Designed by: Yuansi He   Group: iGEM20_SYSU-CHINA   (2020-10-17)
Revision as of 18:22, 25 October 2020 by Ysi He (Talk | contribs)

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The ribozyme sequence and ligation sequence added at both ends of dsRNA facilitate cyclization after


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 78
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 78
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 159
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 78
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 78
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If you want to use this system in non-mammalian cells such as yeast cells, you may need to adjust the sequence of both ends according to the endogenous enzymes


Source

References

Jacob L. Litke 1,2 and Samie R. Jaffrey, Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts, Nature biotechnology, 2018