Composite

Part:BBa_K3408011

Designed by: Yunong Li   Group: iGEM20_NAU-CHINA   (2020-10-09)
Revision as of 14:33, 16 October 2020 by NJAU-Chappie (Talk | contribs)

PliaG-B0034-lacI-Pgrac-B0034-CⅠ-PCⅠ-GFP-B0015

This composite part consists of promoter PliaG and Pgrac, lacI gene, CⅠ gene, GFP gene and some essential RBS and terminators. Promoter PliaG starts transcription process and its transcription production is LacI, which is regulated by IPTG. When bacteria is exposed to medium which is rich in IPTG, bacteria can intake IPTG. IPTG is combined with LacI, then LacI cannot be bound to promoter Pgrac which is specially suppressed by LacI. Therefore, CⅠ repressor protein cannot generate as downstream cⅠ gene cannot transcribe regularly.

As a result, PCⅠ recovers its activity and triggers the downstream transcription of GFP. By checking the expression of GFP, we could verify the feasibility of IPTG induction system, thus guaranteeing the successful culture of our engineered Bacillus subtilis.

Plasmid profile T--NAU-CHINA--composite-parts6.1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2155


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Categories
Parameters
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