Composite

Part:BBa_K3570002:Design

Designed by: Anton Mykhailiuk   Group: iGEM20_Toulouse_INSA-UPS   (2020-10-15)
Revision as of 16:36, 15 October 2020 by Antonmykhailiuk (Talk | contribs)

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Provitamin A synthesis from GGPP in S. cerevisiae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1297
    Illegal PstI site found at 2357
    Illegal PstI site found at 2627
    Illegal PstI site found at 3605
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2012
    Illegal NheI site found at 5006
    Illegal PstI site found at 2357
    Illegal PstI site found at 2627
    Illegal PstI site found at 3605
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 738
    Illegal BglII site found at 3364
    Illegal BglII site found at 5489
    Illegal BglII site found at 6585
    Illegal BamHI site found at 3157
    Illegal XhoI site found at 4943
    Illegal XhoI site found at 4984
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1297
    Illegal PstI site found at 2357
    Illegal PstI site found at 2627
    Illegal PstI site found at 3605
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1297
    Illegal PstI site found at 2357
    Illegal PstI site found at 2627
    Illegal PstI site found at 3605
    Illegal NgoMIV site found at 2018
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized for S. cerevisiae


Source

HO HA up/down - S. cerevisiae genome CrtI - X. dendrorhous genome CrtYB - X. dendrorhous genome Promoter TDH1 - S. cerevisiae genome Terminator cyc1 - S. cerevisiae genome URA3 selective locus - S. cerevisiae genome

References