Composite

Part:BBa_K3487012

Designed by: RuiXin Lin   Group: iGEM20_SZPT-CHINA   (2020-10-14)
Revision as of 13:25, 18 October 2020 by LiuWanTing (Talk | contribs)


Histidine kinase receptor with leader peptide

Description

comD, as the sensory regulatory membrane protein in the two-component regulatory system of Streptococcus mutans, is very important in the function of transmitting signals. So in order to adapt comD to E. coli, we added a leader peptide before comD to help the expressed membrane protein comD attach to the cell membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 120
    Illegal AgeI site found at 595
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

【1】Extracellular expression of Thermobifida fusca cutinase with pelB signal peptide depends on more than type II secretion pathway in Escherichia coli[J]. Journal of Biotechnology, 2015, 204:47-52. 【2】陈冰, 王金华, 陈娇,等. ComD基因缺陷对变异链球菌生物膜形成的影响[J]. 中国微生态学杂志, 2015, 27(009):1034-1037.

[edit]
Categories
Parameters
None