RBS

Part:BBa_K3425031:Design

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-13)
Revision as of 10:26, 14 October 2020 by Tereza H (Talk | contribs)


iGEM Type IIS pSB3C11-DY


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The spacer provides aligned spacing (the distance between RBS and ATG) of 9 nucleotides when Type IIS iGEM Standard assembly is used.

Source

iGEM Registry of Standard Parts BBa_B0032

References

For aligned spacing refer:

Ma, J., Campbell, A., and Karlin, S. (2002) Correlations between Shine-Dalgarno Sequences and Gene Features Such as Predicted Expression Levels and Operon Structures. J. Bacteriol. 184, 5733–5745