DNA

Part:BBa_K3570009

Designed by: Anton Mykhailiuk   Group: iGEM20_Toulouse_INSA-UPS   (2020-10-01)
Revision as of 04:49, 13 October 2020 by Antonmykhailiuk (Talk | contribs)


HO upstream homologous sequence

Usage

HO upstream homology arm part shall be used together with HO downstream homology arm part (BBa_K3570010) to form a functional yeast integration locus. When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene.

Experiments

Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.

Source

  • [1]S. cerevisiae genome, chromosome IV, HO gene. GenBank: CP046084.1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None