Coding
Part:BBa_K3570008
Designed by: Anton Mykhailiuk Group: iGEM20_Toulouse_INSA-UPS (2020-09-30)
Revision as of 18:56, 12 October 2020 by Antonmykhailiuk (Talk | contribs)
HIS3 selection marker
Usage
HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyzes the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli. HIS3 gene serves as a commonly used yeast selectable marker. When HIS3 gene is inserted into an integrative or replicative plasmid, it selects and maintains a yeast vector in yeast host cells. Those cells should not have the functional HIS3 gene in its genome[1].
The sequence contains HIS3 specific promoter, HIS3 coding sequence, and HIS3 terminator.
Source
- [1]Old, R. W., & Primrose, S. B. (1981). Principles of gene manipulation: an introduction to genetic engineering (Vol. 2). Univ of California Press.
- [2]GenBank: U03439.1
- [3]RS313 plasmid https://www.addgene.org/vector-database/3972/
- [4]https://web.archive.org/web/20060505213339/http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1005
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 896
Illegal BglII site found at 956 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |