Coding

Part:BBa_K3629010:Design

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-12)
Revision as of 05:32, 12 October 2020 by Sravyakakumanu (Talk | contribs)

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Neocallimastix patriciarum BGS with 6X His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 104
    Illegal BglII site found at 2085
    Illegal BamHI site found at 417
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 928
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a long gene that might have to be split in two parts when ordered from a DNA synthesizing company. It was also codon optimized for expression and function in Yarrowia lipolytica.


Source

From genomic sequence of Neocallimastix patriciarum.

References