Regulatory

Part:BBa_K3629001:Design

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-09)
Revision as of 02:58, 19 October 2020 by Sravyakakumanu (Talk | contribs) (→‎Design Notes)


Yarrowia lipolytica TEF1 promoter with an intron


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The wild-type sequence of this promoter contains BsaI, PstI, and SpeI restriction sites in the promoter section before the intron sequence. Therefore, single-nucleotides changes that match the ones in the functional BBa_K2983050 promoter were made to remove these sites.

Source

From the genomic sequence of wild-type Y. lipolytica strain CLIB122.

References