Coding
Part:BBa_K3629009
Designed by: Sravya Kakumanu Group: iGEM20_Calgary (2020-10-12)
Trichoderma reesei EGII with FLAG tag
Endoglucanase II coding sequence from Trichoderma reesei with FLAG tag.
Usage and Biology
Design
The native signal peptide from T. reesei was removed so it would not interfere with fused secretion tags native to Y. lipolytica
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 283
Illegal NgoMIV site found at 817
Illegal NgoMIV site found at 1135 - 1000COMPATIBLE WITH RFC[1000]
References
[edit]
Categories
Parameters
//awards/part_collection
//chassis/eukaryote/yeast
//chassis/eukaryote/yeast
None |