Coding

Part:BBa_K3629006

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-11)
Revision as of 02:43, 19 October 2020 by Sravyakakumanu (Talk | contribs) (→‎Design)


Neurospora crassa CBHI with Myc Tag

Cellobiohydrolase I coding sequence from Neurospora crassa with 6x Myc tag.

Usage and Biology

Design

The native signal peptide from N. crassa was removed so it would not interfere with fused secretion tags native to Y. lipolytica

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 585
    Illegal BglII site found at 945
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1453
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Guo Z-P, Duquesne S, Bozonnet S, Cioci G, Nicaud J-M, Marty A, O’Donohue MJ. Conferring cellulose-degrading ability to Yarrowia lipolytica to facilitate a consolidated bioprocessing approach. Biotechnology for Biofuels. 2017;10(1). doi:10.1186/s13068-017-0819-8


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Categories
Parameters
None