Coding

Part:BBa_K3629005

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-11)
Revision as of 02:42, 19 October 2020 by Sravyakakumanu (Talk | contribs) (→‎Design)


Modified Penicillium funiculosum CBHI with 6X His tag

Temperature and pH optimized cellobiohydrolase I coding sequence from Penicillium funiculosum with 6x HIS tag.

Usage and Biology

Design

The native signal peptide from P. funiculosum was removed so it would not interfere with fused secretion tags native to Y. lipolytica.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 564
    Illegal BamHI site found at 1048
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[edit]
Categories
Parameters
None