Protein_Domain

Part:BBa_K3629003

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-10)
Revision as of 04:12, 12 October 2020 by Sravyakakumanu (Talk | contribs)


Yarrowia lipolytica TEF1 promoter with first half of Lip2 signal peptide

TEF1 promoter from Yarrowia lipolytica and the first 42nts (14 codons) of the Lip2 signal peptide (BBa_K1592000)from Y. lipolytica


Usage and Biology

The part contains wild-type TEF1 promoter from Yarrowia lipolytica and the first 42nts (14 codons) of the Lip2 signal peptide from Y. lipolytica (BBa_K1592000)

Design

Upon digestion with BbsI and this part can be assembled into a destination vector (containing BBa_K3629012) with a coding sequence that contains the last 57nts (19 codons) of the Lip2 signal peptide at the 5' end by using Gibson Assembly. In our part collection, this part can be assembled with ________ into the BBa_K3629012 destination vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 53
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

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Categories
Parameters
None