Coding
Part:BBa_K3606003:Design
Designed by: Mingwei Li Group: iGEM20_Fudan (2020-10-11)
mcbABCD
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2297
Illegal PstI site found at 2330 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2297
Illegal PstI site found at 2330 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2297
Illegal PstI site found at 2330 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2297
Illegal PstI site found at 2330
Illegal NgoMIV site found at 1959
Illegal AgeI site found at 2131 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is an antibiotic coding gene cluster with leader peptide and proteins for maturation. We introduced it to our improved antibiotic expressing system to inhibit the growth of other bacteria in human intestine so as to enhance Nissle's competitiveness,as well as to reduce risk of illness caused by some opportunistic pathogens.
Source
We got the plasmid containing this part and other genes from Fudan 2019