Part:BBa_K3505006:Design

Tyr1-AIDAc Tyrosinase fused to membrane protein AIDAc. GB compatible B3-B5

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1439
  Illegal EcoRI site found at 2135
  Illegal XbaI site found at 1043
  Illegal PstI site found at 1487
  Illegal PstI site found at 2073
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1439
  Illegal EcoRI site found at 2135
  Illegal PstI site found at 1487
  Illegal PstI site found at 2073
  Illegal NotI site found at 1211
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1439
  Illegal EcoRI site found at 2135
  Illegal BglII site found at 471
  Illegal BamHI site found at 454
  Illegal BamHI site found at 2337
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1439
  Illegal EcoRI site found at 2135
  Illegal XbaI site found at 1043
  Illegal PstI site found at 1487
  Illegal PstI site found at 2073
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1439
  Illegal EcoRI site found at 2135
  Illegal XbaI site found at 1043
  Illegal PstI site found at 1487
  Illegal PstI site found at 2073
  Illegal AgeI site found at 533
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is present in pUPD2 as Level 0 and has overhangs compatible for Golden Braid cloning. The CDS has position B3-B5.

Source

• Tyr1 from Rhizobium etli in the supplementary of [3]
• AIDA from E.coli in the supplementary of [2]

References

• [1]
• [2]
• [3]