Composite

Part:BBa_K3408009

Designed by: Yunong Li   Group: iGEM20_NAU-CHINA   (2020-10-08)
Revision as of 14:02, 16 October 2020 by NJAU-Chappie (Talk | contribs)

PliaG-trigger RNA-B0015-PCⅠ-switch RNA-GFP-B0015

We used constitutive promoter PliaG to transcribe trigger RNA all the time. And we also used PCⅠ as a constitutive promoter to transcribe switch RNA. If our Toehold switch could work normally, GFP could be translated and we could see green bacteria in our culture medium.

Plasmid profile T--NAU-CHINA--composite-parts4.1.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 239
    Illegal BglII site found at 461
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1190


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