Part:BBa_K3593009

ssDNA, aptamer for β-amanitin

The aptamer we selected by magnetic beads SELEX for β-amatoxin. Its property is yet to be verified due to the negative result obtained from ELONA

Background

Amatoxins are chemicals present inside the genus Amanita and caused about 90% of mushroom poisoning. Being able to detect it before eating or in the field could possibly make a great decrease in people and animals who died because of poisonous mushrooms. Also being able to detect it in hospital can greatly help doctors in mushroom areas get correct information and do effective diagnosis to save the patient.

Sequence and features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterisation of this part

Design

Aptamers are oligonucleotides that form secondary structures, giving them the ability to bind targeted molecules, including ions or small molecules, and, in our case, amanitin.

Experiment data

BBa_K3593009 is an aptamer for β-amanitin, which is selected by us from 15 rounds of SELEX process, along with BBa_K3593007and BBa_K3593008. Its binding affinity is yet to be verified.