Coding

Part:BBa_I757007

Designed by: Freiburg iGEM 2007 team   Group: iGEM07_Freiburg   (2007-10-24)
Revision as of 14:18, 7 October 2020 by Antonmykhailiuk (Talk | contribs)

phyA1-406_N_part


Toulouse_INSA-UPS 2020contributed to the characterisation of this part by adding a new documentation learned form literature on how to use Fhy1 and PhyA as a transcriptoinal optogenetic effectors.
(--antonmykhailiuk 15:26, 07 October 2020 (UTC+2))

  • PhyA is an interaction partner of Fhy1 (parts BBa_I757002, I757004)
  • Purpose: design of a light switchable interaction
  • this part codes for Amino acids 1-406, encompassing the potential Fhy1-interaction domain
  • N-terminus of this protein should not be fused for fusions to avoid disturbance of folding and potential Fhy1 interaction ("N-part")
  • SwissProt: P14712
  • NgoMIV / AgeI protein fusion part
  • iGEM Team Freiburg 2007
  • synthetic DNA by GeneArt optimized for E.coli
  • Part in pGA4 vector (AmpR, ColEI ori)

Contribution from other teams

Toulouse_INSA-UPS 2020's contribution

Characterisation

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1219
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None