Composite

Part:BBa_K3519010:Design

Designed by: Omkar Mohapatra   Group: iGEM20_IISER-Tirupati_India   (2020-10-07)
Revision as of 13:40, 7 October 2020 by Omkarmohapatra123 (Talk | contribs) (→‎References)

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araC-araBAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1043
    Illegal NheI site found at 1066
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1388
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1223
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1205


Design Notes

The part is RFC 10 compatible.


Source

https://2015.igem.org/Team:Glasgow https://parts.igem.org/Part:BBa_K2442101

References

Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995;177(14):4121-4130. doi:10.1128/jb.177.14.4121-4130.1995