Composite

Part:BBa_K3519009

Designed by: Omkar Mohapatra   Group: iGEM20_IISER-Tirupati_India   (2020-10-07)
Revision as of 11:01, 24 October 2020 by Omkarmohapatra123 (Talk | contribs)


medium promoter-traT

This part has the traT gene (BBa_K3519003) downstream to the medium strength Anderson promoter (BBa_J23118), the strong RBS (BBa_B0034) and is followed by the double terminator (BBa_B0015). Constitutive overexpression of the traT gene helps reduce the formation of stable mating pairs.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 703
    Illegal BsaI.rc site found at 185


[edit]
Categories
Parameters
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