Coding

Part:BBa_K3519000:Design

Designed by: Omkar Mohapatra   Group: iGEM20_IISER-Tirupati_India   (2020-10-07)
Revision as of 10:16, 7 October 2020 by Omkarmohapatra123 (Talk | contribs) (→‎Design Notes)

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sulX


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 23
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
    Illegal AgeI site found at 576
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is RFC 10 compatible. This part has not been codon optimised.

Source

Microbacterium sp. CJ77 sulX gene for sulfonamide monooxygenase SulX, complete CDS

References

Kim DW, Thawng CN, Lee K, Wellington EMH, Cha CJ. A novel sulfonamide resistance mechanism by two-component flavin-dependent monooxygenase system in sulfonamide-degrading actinobacteria. Environ Int. 2019 Jun;127:206-215. doi: 10.1016/j.envint.2019.03.046. Epub 2019 Mar 28. PMID: 30928844.