Intermediate

Part:BBa_S04059

Designed by: Doug Tischer   Group: Guests   (2008-08-17)
Revision as of 18:34, 6 October 2020 by Wilson-Li (Talk | contribs)


katG (LAA).B0015

Construction intermediate

Characterization by team Mingdao 2020

ldhp-AQP-RBS-SpxB-Tr-tpxp-KatG-Tr/pSB1C3 (BBa_K3376012)
Catalase converts H2O2 to O2 and H2O preventing cells from oxidative stress. When E. coli Nissle strain (EcN) was expressing aquaporin (AQP) and pyruvate oxidase (SpxB) to produce H2O2, the cell growth was inhibited remarkably compared to a vector-only control. By adding of catalase (KatG), the growth rate became normal and have similar response to glucose concentrations with the control.
T--Mingdao--ww5.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 228
    Illegal BglII site found at 1163
    Illegal BglII site found at 1290
    Illegal BamHI site found at 1080
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None