Coding

Part:BBa_K3376014

Designed by: Li, Sheng-Fong   Group: iGEM20_Mingdao   (2020-10-05)
Revision as of 04:50, 6 October 2020 by Wilson-Li (Talk | contribs)


Aequorea victoria GFPmut1

This part was modified from GFPmut1 (Part:BBa_K1159311) designed by TU-Munich in iGEM 2013 by adding ATG and a stop codon for protein expression.

Expression

GFP was detected at Ex/Em = 483/513. The fluorescence expression levels were measured for lactate dehydrogenase promoter (ldhp) [ldhp-GFP-Tr/pSB1C3 (BBa_K3376002)] and thiol peroxidase promoter (tpxp)[tpxp-GFP-Tr/pSB1C3 (BBa_K3376004)] activity of S. mutans.

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Transformation

The STM1128 gene was amplified from gDNA of Salmonella typhimurium and cloned onto pSB1C3. This part has been sequenced.

Mingdaophil1026-3.jpeg




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


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Categories
Parameters
None