Coding

Part:BBa_K3610021

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-10-04)
Revision as of 19:35, 4 October 2020 by Jtrott (Talk | contribs)


mCherry C-terminal - codon optimized for S. cerevisiae

This part is a modified version of Part:BBa_K1093017. It has been codon optimized for expression in S. cerevisiae and additionally the split was made at amino acids 158 and 159.

Usage and Biology

mCherry is a monomeric red fluorescent protein that has been used for observing components by fusing the sequence to a protein of interes and observing the mCherry by flourescent spectroscopy and microscopy. The mCherry protein is the basis for the bimolecular fluorescence fusion technique which is used to visualize protein-protein interaction. The mCherry is split into two parts, in our case between amino acids 158 and 159. The two components can be fused to proteins of interest and, upon dimerization, are reconstituted to the fully functional red fluorescent protein (Excitation/Emission wavelengths: 587/610 nm).

This part contains the C-terminal part of the split mCherry system and can be used together with Part:BBa_K3610018 for investigation of protein-protein interaction in S. cerevisiae.

In our project, we used this part for visualizing the interaction of leucine-rich repeat receptor-like kinases (LRR-RLKs). We fused the protein to the ectodomains of plant pattern-recognition recpetors which need dimerization to initiate an immune response in plants.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None