Reporter

Part:BBa_K3328031:Design

Designed by: Qi Wu   Group: iGEM20_OUC-China   (2020-10-03)
Revision as of 12:46, 27 October 2020 by Txzz (Talk | contribs) (→‎Design Notes)

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GFPmut3b-ASV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

GFPmut3b protein with ASV-tagged whose half-life is ~110 min

Source

synthesize from company


References

Kim, J., Zhou, Y., Carlson, P. D., Teichmann, M., Chaudhary, S., Simmel, F. C., … Green, A. A. (2019). De novo-designed translation-repressing riboregulators for multi-input cellular logic. Nature Chemical Biology. doi:10.1038/s41589-019-0388-1