Part:BBa_K3570018
MET17 selection marker
The MET17 gene, found in the Saccharomyces cerevisiae yeast, encodes a bifunctional enzyme with O-acetylserine and O-acetylhomoserine sulfhydrylase activities involved in methionine and cysteine metabolism; catalyses the reaction between acetylated serine or homoserine with thiol to produce the corresponding amino acids; localized to the cytoplasm and plasma membrane.
When inserted into an integrative or replicative plasmid, MET17 allows to counter-select the cells that acquired the wanted plasmid.
References: 1- SGD:S000004294
2- Masselot, M., & de Robichon-Szulmajster, H. (1975). Methionine biosynthesis in Saccharomyces cerevisiae. Molecular and General Genetics MGG, 139(2), 121–132. https://doi.org/10.1007/bf00264692
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 649
Illegal XbaI site found at 378 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 649
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 649
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 649
Illegal XbaI site found at 378 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 649
Illegal XbaI site found at 378
Illegal NgoMIV site found at 268 - 1000COMPATIBLE WITH RFC[1000]
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