Coding

Part:BBa_K3588003

Designed by: Louis Gringras, Yashaar Daad   Group: iGEM20_CLS_CLSG_UK   (2020-10-03)
Revision as of 16:31, 3 October 2020 by Mrlouisg (Talk | contribs)


Cocaine Esterase mutant enzyme (E172-173)

The mutant enzyme of part (part:BBa_K3588001). It is able to breakdown cocaine, as well as benzoylecogonine, through hydrolysis into benzoate and ecgonine methyl ester. With a dramatically increased in vitro half life from the wildtype enzyme it is currently in phase II trials as a cocaine overdose treatment,[1] though in our project we were intending to use it to breakdown cocaine in the sewers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1179
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. ↑ https://pubs.acs.org/doi/abs/10.1021/cb500257s
[edit]
Categories
//function/degradation
Parameters
None