Primer
T7
Part:BBa_G00113
Designed by: Reshma Shetty Group: iGEM07_Example (2008-10-17)
T7 promoter sequencing primer, 20-mer
This primer anneals to the T7 Promoter region of any vector containing the T7 Promoter.
Usage and Biology
Contribution: New documentation from Evry_Paris-Saclay 2020
This part is the strong promoter from T7 bacteriophage (taatacgactcactata) with GGG at 3' end.
The T7 RNA polymerase initiates the transcription at the first guanidine of this stretch of three G and it was shown that +1 GGG is one of the best +1, +2 and +3 base combinations at the transcription initiation for enhanced promoter strength [1].
References
[1] Imburgio D, Rong M, Ma K, McAllister WT. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry (2000) 39, 10419-10430.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//primer/T7
//chassis/bacteriophage/T7
//chassis/bacteriophage/T7
| direction | F |
| len_end | |
| len_fr | |
| len_start | |
| pct_gc | 40.0 |
| target | T7 promoter |
| t_m | 47.5 |