Other
TIIS RBS

Part:BBa_K3425018

Designed by: Núria Garriga Alonso, Björn Ancker Persson, Tereza Hubáčková, Dorottya Marko, Hui Yu   Group: iGEM20_UofUppsala   (2020-10-03)
Revision as of 12:23, 3 October 2020 by Tereza H (Talk | contribs)


iGEM Type IIS standard RBS template

This part is the strong constitutive promoter BBa_J23104 adopted to the iGEM Type IIS standard (RFC1000). The BBa_J23104 sequence is flanked by the Type IIS prefix and suffix which allows cloning into the Level 0 backbone (pSB1C00). BBa_J23104 is part of the Anderson promoter family and its relative strength is 1. BBa_K3425018 can be ordered as two overlapping primers, see iGEM Type IIS guide of Uppsala iGEM 2020.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 5
    Illegal SapI.rc site found at 39

Note: The part contains restriction sites for cloning into Level 0 universal Type IIS acceptor pSB1C00, therefore it is marked as not compatible with RFC1000 standard.

[edit]
Categories
//collections/typeiis/uppsala
//collections/typeiis/uppsala/templates
Parameters
None