Composite

Part:BBa_K3349008:Design

Designed by: Kristi Turton   Group: iGEM20_Lethbridge_HS   (2020-09-28)
Revision as of 15:21, 7 October 2020 by Kristiturton (Talk | contribs) (→‎Design Notes)

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Pectin Lyase PelC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 901
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 287


Design Notes

This part was codon optimized for use in E. coli. A C-terminal His tag was added based on previous pectin lyase research [2].

Source

This part was originally taken from the uniprot database. The NCBI sequence ID is WP_123062310.1.

References

1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of Paenibacillus amylolyticus 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.

2. Mie, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purification, and biochemical properties of a thermostable pectinase Bacillus halodurans M29. Journal of Molecular catalysis B: Enzymatic. 94, 77-81