Coding

Part:BBa_K3610005:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-08-24)
Revision as of 13:06, 3 October 2020 by Jtrott (Talk | contribs) (→‎References)

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EFR from A. thaliana


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2753
    Illegal XbaI site found at 433
    Illegal XbaI site found at 2568
    Illegal SpeI site found at 1114
    Illegal PstI site found at 2407
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2753
    Illegal SpeI site found at 1114
    Illegal PstI site found at 2407
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2753
    Illegal BglII site found at 2418
    Illegal BglII site found at 2446
    Illegal BglII site found at 2989
    Illegal XhoI site found at 2442
    Illegal XhoI site found at 3032
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2753
    Illegal XbaI site found at 433
    Illegal XbaI site found at 2568
    Illegal SpeI site found at 1114
    Illegal PstI site found at 2407
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2753
    Illegal XbaI site found at 433
    Illegal XbaI site found at 2568
    Illegal SpeI site found at 1114
    Illegal PstI site found at 2407
    Illegal AgeI site found at 256
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None

Source

Cyril Zipfel Lab at UZH, Switzerland

References

Macho, Alberto P.; Zipfel, Cyril (2014): Plant PRRs and the Activation of Innate Immune Signaling. In: Molecular Cell 54 (2), S. 263–272. DOI: 10.1016/j.molcel.2014.03.028.

Yan, Liming; Ma, Yuanyuan; Liu, Dan; Wei, Xiaochao; Sun, Yuna; Chen, Xiaoyue et al. (2012): Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1. In: Cell Res 22 (8), S. 1304–1308. DOI: 10.1038/cr.2012.74.

Chinchilla, Delphine; Shan, Libo; He, Ping; Vries, Sacco de; Kemmerling, Birgit (2009): One for all: the receptor-associated kinase BAK1. In: Trends Plant Sci 14 (10), S. 535–541. DOI: 10.1016/j.tplants.2009.08.002.

Pfeilmeier, Sebastian; George, Jeoffrey; Morel, Arry; Roy, Sonali; Smoker, Matthew; Stransfeld, Lena et al. (2019): Expression of the Arabidopsis thaliana immune receptor EFR in Medicago truncatula reduces infection by a root pathogenic bacterium, but not nitrogen-fixing rhizobial symbiosis. In: Plant biotechnology journal 17 (3), S. 569–579. DOI: 10.1111/pbi.12999.

Kunze, Gernot; Zipfel, Cyril; Robatzek, Silke; Niehaus, Karsten; Boller, Thomas; Felix, Georg (2004): The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants. In: Plant Cell 16 (12), S. 3496–3507. DOI: 10.1105/tpc.104.026765.