Part:BBa_K3610004:Design
BAK1 ectodomain from Arabidopsis thaliana - codon optimized for C. reinhardtii
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 778
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 708
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized for C. reinhardtii. This sequence doesn't contain codons with a occurrence probability lower than 10%.
The juxtamembrane domain must be included in the sequence since the interaction with other plant receptors has shown to be weaker without this domain.
Source
Cyril Zipfel Lab at UZH, Switzerland Codon optimized with SnapGene
References
Chinchilla, Delphine; Zipfel, Cyril; Robatzek, Silke; Kemmerling, Birgit; Nürnberger, Thorsten; Jones, Jonathan D. G. et al. (2007): A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. In: Nature 448 (7152), S. 497–500. DOI: 10.1038/nature05999.
Macho, Alberto P.; Zipfel, Cyril (2014): Plant PRRs and the Activation of Innate Immune Signaling. In: Molecular Cell 54 (2), S. 263–272. DOI: 10.1016/j.molcel.2014.03.028.
Yan, Liming; Ma, Yuanyuan; Liu, Dan; Wei, Xiaochao; Sun, Yuna; Chen, Xiaoyue et al. (2012): Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1. In: Cell Res 22 (8), S. 1304–1308. DOI: 10.1038/cr.2012.74.
Chinchilla, Delphine; Shan, Libo; He, Ping; Vries, Sacco de; Kemmerling, Birgit (2009): One for all: the receptor-associated kinase BAK1. In: Trends Plant Sci 14 (10), S. 535–541. DOI: 10.1016/j.tplants.2009.08.002.
Zhou, Qi; Liu, Jun; Wang, Jingyi; Chen, Sufen; Chen, Lijuan; Wang, Jinfa et al. (2020): The juxtamembrane domains of Arabidopsis CERK1, BAK1, and FLS2 play a conserved role in chitin-induced signaling. In: Journal of integrative plant biology 62 (5), S. 556–562. DOI: 10.1111/jipb.12847.
Naya, Hugo; Romero, Héctor; Carels, Nicola; Zavala, Alejandro; Musto, Héctor (2001): Translational selection shapes codon usage in the GC-rich genome of Chlamydomonas reinhardtii. In: FEBS Letters 501 (2), S. 127–130. DOI: 10.1016/S0014-5793(01)02644-8.