Coding

Part:BBa_K3610003:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-09-01)
Revision as of 13:01, 3 October 2020 by Jtrott (Talk | contribs) (References)


BAK1 Ectodomain from Arabidopsis thaliana


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 576
    Illegal PstI site found at 621
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 576
    Illegal PstI site found at 621
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 576
    Illegal PstI site found at 621
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 576
    Illegal PstI site found at 621
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The juxtamembrane domain must be included in the sequence since the interaction with other plant receptors has shown to be weaker without this domain.

Source

Cyril Zipfel Lab at UZH, Switzerland

References

Chinchilla, Delphine; Zipfel, Cyril; Robatzek, Silke; Kemmerling, Birgit; Nürnberger, Thorsten; Jones, Jonathan D. G. et al. (2007): A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. In: Nature 448 (7152), S. 497–500. DOI: 10.1038/nature05999.

Macho, Alberto P.; Zipfel, Cyril (2014): Plant PRRs and the Activation of Innate Immune Signaling. In: Molecular Cell 54 (2), S. 263–272. DOI: 10.1016/j.molcel.2014.03.028.

Yan, Liming; Ma, Yuanyuan; Liu, Dan; Wei, Xiaochao; Sun, Yuna; Chen, Xiaoyue et al. (2012): Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1. In: Cell Res 22 (8), S. 1304–1308. DOI: 10.1038/cr.2012.74.

Chinchilla, Delphine; Shan, Libo; He, Ping; Vries, Sacco de; Kemmerling, Birgit (2009): One for all: the receptor-associated kinase BAK1. In: Trends Plant Sci 14 (10), S. 535–541. DOI: 10.1016/j.tplants.2009.08.002.

Zhou, Qi; Liu, Jun; Wang, Jingyi; Chen, Sufen; Chen, Lijuan; Wang, Jinfa et al. (2020): The juxtamembrane domains of Arabidopsis CERK1, BAK1, and FLS2 play a conserved role in chitin-induced signaling. In: Journal of integrative plant biology 62 (5), S. 556–562. DOI: 10.1111/jipb.12847.